Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1972 Nov;24(5):772–777. doi: 10.1128/am.24.5.772-777.1972

An Unclassified Gram-Negative Rod Isolated From the Pharynx on Thayer-Martin Medium (Selective Agar)

Dannie G Hollis 1, Geraldine L Wiggins 1, Robert E Weaver 1
PMCID: PMC380662  PMID: 4565636

Abstract

An oxidase-positive, small gram-negative rod was isolated on Thayer-Martin medium (TM) inoculated with pharyngeal swabs obtained during surveys to detect Neisseria carriers. In one survey, this organism was isolated from 48% of the subjects, and 50 or more colonies were present on the majority of the primary isolation plates. Other characteristics of the organism, which has been given the provisional designation „TM-1,” include: delayed production (2 to 10 days) of acid from glucose, formation of gas during nitrate reduction, and the frequent formation of „pits” in the agar surface. On TM, nonpitting colonies of TM-1 are morphologically similar to colonies of Neisseria gonorrhoeae and N. lactamica. Comparison of the characteristics of TM-1 strains with other similar fastidious gram-negative organisms encountered in clinical laboratories indicates that TM-1 is a distinct species. Further studies are required before proper taxonomic placement can be made.

Full text

PDF
776

Images in this article

773Image
on p.773

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Henriksen S. D., Bovre K. Moraxella kingii sp.nov., a haemolytic, saccharolytic species of the genus Moraxella. J Gen Microbiol. 1968 May;51(3):377–385. doi: 10.1099/00221287-51-3-377. [DOI] [PubMed] [Google Scholar]
  3. Henriksen S. D. Corroding bacteria from the respiratory tract. 1. Moraxella kingii. Acta Pathol Microbiol Scand. 1969;75(1):85–90. [PubMed] [Google Scholar]
  4. Henriksen S. D. Corroding bacteria from the respiratory tract. 2. Bacteroides corrodens. Acta Pathol Microbiol Scand. 1969;75(1):91–96. [PubMed] [Google Scholar]
  5. Hill L. R., Snell J. J., Lapage S. P. Identification and characterisation of Bacteroides corrodens. J Med Microbiol. 1970 Aug;3(3):483–491. doi: 10.1099/00222615-3-3-483. [DOI] [PubMed] [Google Scholar]
  6. KING E. O. Studies on a group of previously unclassified bacteria associated with meningitis in infants. Am J Clin Pathol. 1959 Mar;31(3):241–247. doi: 10.1093/ajcp/31.3.241. [DOI] [PubMed] [Google Scholar]
  7. Martin J. E., Jr, Lester A. Transgrow, a medium for transport and growth of Neisseria gonorrhoeae and Neisseria meningitidis. HSMHA Health Rep. 1971 Jan;86(1):30–33. [PMC free article] [PubMed] [Google Scholar]
  8. MØLLER V. Simplified tests for some amino acid decarboxylases and for the arginine dihydrolase system. Acta Pathol Microbiol Scand. 1955;36(2):158–172. doi: 10.1111/j.1699-0463.1955.tb04583.x. [DOI] [PubMed] [Google Scholar]
  9. Pickett M. J., Pedersen M. M. Characterization of saccharolytic nonfermentative bacteria associated with man. Can J Microbiol. 1970 May;16(5):351–362. doi: 10.1139/m70-062. [DOI] [PubMed] [Google Scholar]
  10. SNEATH P. H. Cultural and biochemical characteristics of the genus Chromobacterium. J Gen Microbiol. 1956 Aug;15(1):70–98. doi: 10.1099/00221287-15-1-70. [DOI] [PubMed] [Google Scholar]
  11. Thayer J. D., Martin J. E., Jr Improved medium selective for cultivation of N. gonorrhoeae and N. meningitidis. Public Health Rep. 1966 Jun;81(6):559–562. [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES