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a Immunohistochemical analysis revealed that the tumor cells were positive for HER2 overexpression (Dako, Herceptest). b Dual-color chromogenic in situ hybridization revealed that HER2 gene was highly amplified, with HER2:chromosome 17 centromere ratio at 10:1 or more (Ventana, INFORM HER2 Dual ISH). Red = Chromosome 17 centromere; black = HER2.
