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. Author manuscript; available in PMC: 2013 Oct 23.
Published in final edited form as: J Mol Cell Cardiol. 2012 May 14;53(2):206–216. doi: 10.1016/j.yjmcc.2012.05.003

Figure 1. Mouse cardiomyocytes express prototypical cholinergic markers.

Figure 1

A-C. Representative Western blots of immunoreactivity for VAChT, ChAT, and CHT1 in neonatal and adult mouse ventricular myocytes. Brain samples were used as a positive control. α-tubulin was used as a loading control. D-G. VAChT, FM1-43 FX, ChAT and CHT1 staining in adult mouse ventricular myocytes. Cardiomyocytes were also labeled with antibody against the sarcomeric protein α-actinin to show cellular organization. Scale Bar=10 μm.