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. Author manuscript; available in PMC: 2013 Oct 23.
Published in final edited form as: Science. 2011 Dec 23;334(6063):1713–1716. doi: 10.1126/science.1210770

Figure 1.

Figure 1

A. Diagrams of tagged MSH6 constructs. B. (Top) Expression level of Msh6 revealed by Western blots of whole cell lysates with anti-Myc antibodies for log-phase cells (log), alpha-factor arrested cells (α-F), and cells released from alpha-factor arrest for the indicated times. An anti-Pgk1 blot is shown as a loading control. (Bottom) FACS profiles of cells showing the cell cycle distribution at the indicated time points. The FACS profile displayed is for the MSH6-9MYC msh3Δ strain; however, all of the strains used had essentially identical profiles.