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. 2013 Oct 23;8(10):e76987. doi: 10.1371/journal.pone.0076987

Figure 1. In vitro binding between recombinant human Akt2 truncated proteins and human p21cip1.

Figure 1

A. Pull-down assay was performed between each of three recombinant N-terminal truncated Akt2 proteins (350–481, 400–481, 430–481) and recombinant human p21cip1. Inputs are shown on the left and pull down assays on the right. Membranes were blotted with Akt2 and with p21 as a control. B. Immunoprecipitations (in vitro) were performed between each of four recombinant full length Akt2 (1–481), C-terminal truncated Akt2 proteins (1–460, 1–445 and 1–430) and human recombinant p21cip1. Inputs are shown on the left and pull-down assays on the right. Membranes were blotted with Akt2 and with p21 as a control. C. Pull-down assay was performed between Akt2 peptides (400–445) or (420–445) and recombinant p21cip1. Increasing inputs of p21 (1 µg and 5 µg) are shown on the left lanes and pull-down assays with increasing amounts of p21 on the right lanes. Membranes were blotted with p21 and with MBP as a control.