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. 2013 Oct 23;8(10):e77946. doi: 10.1371/journal.pone.0077946

Figure 5. P1736 promotes glucose uptake by modulating insulin signaling pathways.

Figure 5

a, Effect of P1736 on PI3 - Kinase mediated glucose uptake in insulin resistant 3T3 adipocytes. Insulin resistant adipocytes were treated with P1736 (10 μM) or rosiglitazone (0.1 μM) as mentioned in methods. At the end of 4 days, cells were treated with vehicle (empty bar) or 100 nM wortmannin (solid bar) for 30 minutes. Later 200nmol/l insulin stimulated 2-deoxyglucose uptake was determined. b, Effect of P1736 on GLUT-4 translocation in insulin resistant adipocytes. Insulin resistant 3T3 adipocytes were treated with vehicle or P1736 (10 μM) or Metformin (1 mM) or rosiglitazone (0.1 μM) for 4 days. Later the cells were stimulated with 200nmol/l insulin for 25 minutes. Whole cell immunofluorescence assay for GLUT-4 translocation was performed using rabbit antibody against GLUT - 4 followed by incubation with FITC labeled secondary antibody. Confocal images were obtained using Bio-Rad Laser Scanning Microscope.