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. 2013 Oct 23;8(10):e77960. doi: 10.1371/journal.pone.0077960

Figure 1. Induction of γ-H2AX foci in GV oocytes following MG and resveratrol (RSVL) treatment.

Figure 1

Oocytes were blocked at the GV stage in M2 medium plus 2.5 µM milrinone and were cultured with or without 75 µM MG and different concentration of resveratrol for 6 h. DNA and γ-H2AX were stained and the results show that resveratrol can effectively protect MG-induced the formation of γ-H2AX, the protein marker of DSB. (A) Representative samples of control GV-stage oocytes with γ-H2AX 0–5 foci, >5–10 foci and >10 foci. Red, γ-H2AX; Blue, DNA. Scale bar  = 20 µm. (B) The proportion of GV oocytes with γ-H2AX >10 foci after MG and different concentration of resveratrol treatment (5, 10 and 25 µM). Data are mean ± SEM of three independent experiments. Different letters denote statistically significant differences (P<0.05).