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. 2013 Oct 23;8(10):e78190. doi: 10.1371/journal.pone.0078190

Figure 2. Effect of camptothecin on transcriptional readthrough and synthesis of PROMPTs and eRNA.

Figure 2

As in Figure 1, human fibroblasts were treated with 20 µM camptothecin for 45 min with 2 mM Bru added during the last 15 min of camptothecin treatment to label nascent RNA followed by Bru-Seq. (A), Transcriptional readthrough of the termination site of the RHOB gene induced by camptothecin. (B), Enhanced initiation of the ASCC3 gene and coincident upregulation of divergent upstream PROMPT RNA. (C), Enhanced expression of eRNA from the 5’-upstream enhancer of FOS by camptothecin. (D), Camptothecin inhibits the transcription of the primary transcript of miRNA138-1. (E), Camptothecin induces transcription of the ncRNA MALAT1. (F), Camptothecin inhibits the transcription of a very long unannotated ncRNA on chromosome 2. The gene maps are from RefSeq Genes (UCSC genome browser).