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. 2013 Oct 23;8(10):e79082. doi: 10.1371/journal.pone.0079082

Figure 3. Flow cytometry, cytotoxicity (SADCC) and NFκB-luciferace reporter gene analysis.

Figure 3

A) Analysis of flow cytometry sorted SEA activated T cells and B) cytotoxicity (SADCC) measured against Caki-2 cells with (filled) or without (open) ABR-217620 using a standard 4 hr 51Cr-release assay of unsorted (squares) and sorted (triangles) cells binding the [ABR-217620-biotin/SA-PE]-complex and anti-CD3. C) Activation of NFκB-luciferace reporter gene in J.RT3-T3-5 cells expressing TRBV7-9 by Caki-2 cells and different concentrations of ABR-217620 (squares) or SEA/E-120 (triangles). Activation of the NFκB-luciferace reporter without ABR-217620/SEA/E-120 was equal to the defined 0-value in the graph. D) Activation of NFκB-luciferace reporter gene in J.RT3-T3-5 cells expressing TRBV7-9 by 5T4- and anti-CD2-coated (squares) or control-coated (triangles) beads and different concentrations of ABR-217620. Activation of the NFκB-luciferace reporter without ABR-217620 was equal to the defined 0-value in the graph.