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. 2013 Oct 23;8(10):e78711. doi: 10.1371/journal.pone.0078711

Figure 3. AZT-mediated bystander cell killing is dependent on GJICs and is inhibited by carbenoxolone.

Figure 3

(A) Bystander cell killing was assessed by relative evaluation of annexin V staining in AZT-treated bystander eGFP-expressing PC-3 cells, co-cultured with TMPK-F105Y-tranduced PC-3 cells, by flow cytometry. Co-cultures were either left untreated or treated with the pan GJIC inhibitor, carbenoxolone (CBX) (n=3). (B) Fold-change in the production of reactive oxygen species (ROS) due to AZT treatment was evaluated in wild-type TMPK-expressing and TMPK-F105Y-expressing PC-3 cells (n=3). (C) Fold change in the production of reactive oxygen species (ROS) due to AZT treatment was evaluated in eGFP-expressing PC-3 cells, co-cultured with TMPK-F105Y-tranduced PC-3 cells, with and without carbenoxolone (CBX) treatment (n=3). Statistical significance is indicated (p<0.0001).