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. 2013 Oct 23;8(10):e78635. doi: 10.1371/journal.pone.0078635

Figure 3. Transactivation activity assay of GhERF12 protein in yeast cells.

Figure 3

(A) Yeast transformants were streaked on SD/-Trp medium (SD minimal medium lacking Trp); (B) Yeast transformants were streaked on SD/-Trp/-Ade medium (SD minimal medium lacking Trp and Ade); (C) flash-freezing filter assay of the β-galactosidase activity. 1, Yeast transformants of pGBKT7 was used as the negative control; 2, Yeast transformants of pGBKT7-53/pGADT7-RecT was used as the positive control; 3, Yeast transformants of pGBKT7-GhERF12.