Figure 5. Up-regulation of survival gene expression in leukemia cells from TCL1-Tg:p53−/− mice or from CLL patient samples with 17p deletion.
A Mcl-1, Bcl-XL and Bcl-2 protein expression shown by Western blot analysis (cells isolated from 4-month old mice; n=4 per group).
B Mcl-1 mRNA levels in mouse splenocytes and PC cells shown by RT-PCR. *, p<0.05 between groups (n=4 per indicated strain).
C Bcl-XL mRNA levels in mouse splenocytes and PC cells shown by RT-PCR. *, p<0.05 between groups (n=4 per indicated strain).
D Bcl-2 mRNA levels in mouse splenocytes and PC cells shown by RT-PCR. **, p<0.01 between groups (n=4 per indicated strain).
E Mcl-1, Bcl-XL and Bcl-2 protein levels in CLL cells from human CLL patients with or without 17p deletion (4 >70%17p deletion samples: del1-4; 6 17p WT samples: WT1-6).
F Mcl-1, Bcl-XL and Bcl-2 mRNA levels in CLL cells from patients with or without 17p deletion shown by RT-PCR. **, p<0.01 between groups.