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. Author manuscript; available in PMC: 2014 Jul 19.
Published in final edited form as: ACS Chem Biol. 2013 May 23;8(7):1590–1599. doi: 10.1021/cb400261h

Figure 5.

Figure 5

Competitive and CC-ABPP of clickable carbamate probes in vivo. (a–d) Competitive ABPP of brain (a) and liver (c) membrane proteomes isolated from mice treated with indicated doses of each carbamate (1.0 – 40 mg•kg–1, p.o.). CC-ABPP for the same brain (b) and liver (d) proteomes derived from vehicle- or inhibitor-treated mice. Each inhibitor shows clear labeling of MAGL and ABHD6 in the brain, while FAAH is labeled by JW842yne and, to lesser extent, by MJN110yne. Liver profiles reveal differing degrees of off-target cross-reactivities for each carbamate with JW651yne exhibiting the highest selectivity across the proteome.