Table 1.
Oligonucleotide primers used for RT-qPCR
| Gene | Nucleotide sequence (5’–3’) | Product (pb) | Reference |
|---|---|---|---|
| gapdh | CCCAAAACATCATCCCATCTTCGGAACACGGAACGCCATAC | 60 | 18 |
| ltxA | TTGTCGCAAGTGCCATAGTTATCCACTAGCCCCATGGCAACGGTAGAA | 193 | This study, 22 |
| flp | TCAAAGCAATCGAAGCAATCGCAATAGCGATCAAACCGTA | 82 | This study, 22 |
| aae | GGTTTTAGGCGGCACATTTATGCTTGACCAACCATAACCA | 152 | This study, 22 |
| apaH | CACCTTGGTTTGCCTTGGATATGTCTTCCCAACGTAGCATG | 159 | This study, 22 |
| omp29 | TCTCAACAAGCCATCTCTGCCGACCTTTAACTACGTCGCA | 80 | This study, 22 |
| omp100 | ATCTTCAAGCCAAAACATCAAGGCTGCCGACATTAT | 169 | 11 |
| cdtB | CAACAACACAATTCCAACCCGGCGATACCTGTCCATTCTT | 94 | This study, 22 |
| vapA | CGAAATTATGGCTGGGTATGCAATGGTTGGATGTTGAATACGG | 60 | 31, 22 |
| orf 859 | CAATCTCACCCAAGCCCTACGCGGCGGAAATATAGAAACTG | 83 | 31 |
| vppA | GGTTACCGGTGGAGTTCGCGGGTCGTAATCGTTTGA | 190 | This study, 22 |
| cagE | TGGATTGGGACAAGTGAACATACAAAGCCATAAGAGAAAT | 190 | This study, 22 |
| emaA | CTGCAGCAACCGGGGATTATAATGGATTGGTTGCCTTTAG | 110 | This study, 22 |
| pgA | GACGGTGATGCGGTATTGGGACCGATGATGGAGCTGAA | 160 | 9 |