Table 1.
OFA activity of mice that overexpress human RCAN1a
| Genotype | Time/zone (s) |
Distance/zone (cm) |
Ambulatory (s) |
Resting (s) |
Vertical (s) |
Stereotypic (s) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Periphery | Center | Periphery | Center | Total | Periphery | Center | Total | Periphery | Center | Total | Periphery | Center | Total | Periphery | Center | Total | |
| Nse-RCAN1Tg1a | |||||||||||||||||
| Mean | 767.6 | 132.4 | 1576.2 | 513.9 | 2090.1 | 62.1 | 20.8 | 82.9 | 580.1 | 69.9 | 650 | 92 | 7.6 | 99.6 | 125.3 | 25.3 | 150.6 |
| SEM | 15.5 | 15.5 | 73.4 | 55.6 | 81.7 | 4.3 | 2.4 | 4.4 | 13.1 | 10.3 | 6.1 | 6.7 | 1.7 | 7.8 | 2.6 | 3.3 | 3.1 |
| WT-Tg1a (Nse) | |||||||||||||||||
| Mean | 779.5 | 120.5 | 1596.3 | 510.6 | 2106.9 | 61.7 | 20.8 | 82.5 | 594.9 | 63 | 658 | 98.4 | 6.3 | 104.7 | 122.8 | 22.4 | 145.2 |
| SEM | 10.8 | 10.8 | 99.7 | 60.6 | 128.4 | 4.9 | 2.8 | 6.2 | 12.3 | 6.8 | 9.5 | 8.3 | 1.2 | 9.1 | 3.2 | 2.1 | 3.9 |
| p value | 0.542 | 0.542 | 0.87 | 0.968 | 0.911 | 0.952 | 0.995 | 0.956 | 0.417 | 0.591 | 0.475 | 0.545 | 0.533 | 0.669 | 0.538 | 0.476 | 0.284 |
| Nse-RCAN1Tg1 | |||||||||||||||||
| Mean | 718.5 | 181.5 | 1633.3 | 572.3 | 2205.6 | 69.8 | 19.4 | 89.2 | 549.5 | 101.7 | 651.2 | 63.7 | 5.5 | 69.2 | 114.4 | 29.8 | 144.2 |
| SEM | 35.8 | 35.8 | 92.1 | 78.2 | 140.9 | 9.9 | 3.1 | 11.1 | 29.2 | 27.2 | 16.1 | 9.2 | 2.5 | 10.3 | 4.8 | 5.3 | 6.6 |
| WT-Tg1 (Nse) | |||||||||||||||||
| Mean | 732.1 | 167.9 | 1551.9 | 623.0 | 2174.9 | 63.0 | 28.2 | 91.3 | 536.0 | 106.5 | 642.5 | 44.8 | 3.72 | 48.5 | 115.4 | 35.8 | 151.2 |
| SEM | 37.2 | 37.2 | 74.7 | 88.6 | 105.3 | 9.2 | 5.4 | 10.7 | 27.1 | 29.5 | 14.0 | 8.1 | 0.7 | 8.6 | 7.0 | 7.1 | 3.0 |
| p value | 0.804 | 0.804 | 0.502 | 0.679 | 0.864 | 0.622 | 0.184 | 0.894 | 0.737 | 0.905 | 0.686 | 0.139 | 0.495 | 0.131 | 0.904 | 0.517 | 0.333 |
| CamkIIα-RCAN1Tg1a | |||||||||||||||||
| Mean | 717.8 | 182.2 | 1802.1 | 677.8 | 2479.9 | 73.1 | 27.4 | 100.4 | 536.9 | 85 | 621.9 | 77.4 | 8.9 | 86.3 | 131.9 | 31.6 | 163.5 |
| SEM | 32 | 32 | 97.1 | 75.2 | 128.6 | 5 | 3.1 | 6.3 | 18.4 | 17.2 | 9 | 7.6 | 2.4 | 8.7 | 4.1 | 3.9 | 3.3 |
| WT-Tg1a (CamkIIα) | |||||||||||||||||
| Mean | 746.2 | 153.8 | 1873.5 | 706.8 | 2580.3 | 75.1 | 30 | 105 | 546.7 | 72.7 | 619.4 | 76.4 | 7 | 83.4 | 128.7 | 29.7 | 158.3 |
| SEM | 17.9 | 17.9 | 89.5 | 61.7 | 120.3 | 4.7 | 2.8 | 6.2 | 12.6 | 8.2 | 9 | 7.2 | 1.4 | 8.1 | 2.6 | 2.7 | 3.3 |
| p value | 0.74 | 0.74 | 0.59 | 0.765 | 0.57 | 0.77 | 0.535 | 0.604 | 0.659 | 0.512 | 0.844 | 0.92 | 0.496 | 0.804 | 0.504 | 0.679 | 0.274 |
| CamkIIα-RCAN1Tg1 | |||||||||||||||||
| Mean | 799.5 | 100.5 | 1736.5 | 357.4 | 2093.9 | 70 | 13.3 | 83.3 | 595 | 50.7 | 645.7 | 50.7 | 1.3 | 52 | 134.4 | 18.7 | 153.1 |
| SEM | 13.2 | 13.2 | 123.6 | 43.1 | 137.6 | 6.6 | 1.8 | 7 | 14.8 | 7.7 | 11 | 7.2 | 0.6 | 7.3 | 3.4 | 2.4 | 4.2 |
| WT-Tg1 (CamkIIα) | |||||||||||||||||
| Mean | 822.1 | 77.9 | 1710.1 | 286.1 | 1996.3 | 69.4 | 10.7 | 80.1 | 619.4 | 37.3 | 656.7 | 49.9 | 1.1 | 51 | 133.3 | 15 | 148.3 |
| SEM | 10.5 | 10.5 | 79.4 | 45.2 | 114.1 | 4 | 1.9 | 5.3 | 14.2 | 5.4 | 10.6 | 7 | 0.7 | 7.2 | 3.5 | 1.9 | 4.6 |
| p value | 0.191 | 0.191 | 0.859 | 0.264 | 0.59 | 0.938 | 0.331 | 0.719 | 0.247 | 0.168 | 0.481 | 0.935 | 0.835 | 0.922 | 0.826 | 0.238 | 0.453 |
aTransgenic mice that overexpress human RCAN1 under the control of either a developmental (Nse) or postdevelopmental (CamkIIα) Cre driver show no difference in OFA activity. Two different transgenic RCAN1 lines were used (Oh et al., 2005). No combination of Cre driver and transgenic RCAN1 lines showed any effect on OFA measures used when compared to controls. p value obtained from two-sided independent t test. p Values compare Tg vs WT for each line.