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. 2013 Oct 23;33(43):16945–16960. doi: 10.1523/JNEUROSCI.1445-13.2013

Figure 5.

Figure 5.

CRH-induced spine loss requires NMDA receptor activation, but not AMPA receptor activation. To distinguish the roles of NMDA- and AMPA-type receptors, neurons were exposed to CRH in the presence of selective blockers, APV and CNQX, respectively. A, Example of dendrites exposed to CRH in the presence of APV + CNQX. The combined antagonists prevented CRH-induced spine loss. B, Graph quantifying CRH spine loss in the presence of both glutamate receptor antagonists (F(2,180) = 64.22, p < 0.0001; n = 12). C, Exposing neurons to CRH in the presence of APV or of CNQX revealed that NMDA receptor activation was required for CRH-induced spine loss. D, PSD95 quantification demonstrates that the AMPA receptor blocker CNQX (purple) partially ameliorated the effects of CRH on PSD95-ir puncta (F(2,180) = 38.79, p < 0.0001; post hoc CRH vs CNQX + CRH p > 0.05 at all distance points; n = 12), but did not protect from the effects of CRH on GFP-filled spines (F(2,240) = 43.05, p < 0.0001 n = 12). E, In contrast, NMDA receptor blockade abolished CRH-induced reduction in PSD95 puncta (F(2,180) = 62.78, p < 0.0001; n = 12) and GFP-filled spines (F(2,240) = 34.65, p < 0.0001; n = 12). Scale bars: A, C, 5 μm.