Table 1.
Screening of dyes to monitor mitochondria function
| Dye | MMP sensitive | Single-photon Ex (nm) | Em (nm) | Two-Photon Ex (nm) | In vitro staining |
In vivo staining |
|
|---|---|---|---|---|---|---|---|
| Concentration | Depth | ||||||
| TMRE | Yes | 543 (549*) | 574* | 900 | 0.2 μm | 20 μm | 200 μm |
| MTG | No | 488 (490*) | 516* | 900 | 0.4 μm | 40 μm | 100 μm |
| MTR | Yes | 543 (578*) | 599* | 900 | 0.2 μm | 20 μm | 200 μm |
| DiCO6 | Yes | 488 (484*) | 501* | 900 | 40 nm | 20 μm | 200 μm |
| MitoFluo Red 589 | No | 543 (588*) | 622* | None | 0.2 μm | 10 μm | None |
| Aggregate 543 (535*) | 570* | ||||||
| JC-1 | Yes | Monomer 488 (485*) | 505* | 960 | 10 μg/ml | 100 μg/ml | 50 μm |
*Fluorescent excitation and emission maxima (from Molecular Probes/Invitrogen).