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. 2013 Nov;41(11):1896–1905. doi: 10.1124/dmd.113.053611

Fig. 1.

Fig. 1.

Discontinuous sucrose density gradient profiles of enzyme-lipid reconstituted systems after detergent solubilization with Brij 98. Reconstituted systems containing CYP1A2 and/or CPR (as indicated in the center of the figure) were prepared and solubilized with Brij 98 (at the concentrations indicated) as described in Materials and Methods. The resulting suspensions were layered on a discontinuous sucrose gradient, and 1-ml fractions from the top to bottom of the gradient (indicated by the lane numbering) were analyzed by Western blotting for either CPR or CYP1A2. The asterisk indicates the lane location of the 50-and 70-kDa molecular weight marker for CYP1A2 and CPR, respectively. (A) Profiles generated from V-PC reconstituted systems, and (B) profiles generated from V-ER systems. When both enzymes were included in the reconstituted system, the word “Both” is used, and the enzyme detected in the Western blots is indicated in parentheses. The results are representative of at least three separate experiments.