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FRET interaction of NBD-PE and Rh-PE in lipid vesicles mimicking the ER membrane. Degree of FRET interaction was calculated as stated in Materials and Methods for vesicles composed of natural lipids mimicking the ER membrane. Measurements were done at 23°C (A) and 37°C (B). These data represent the mean ± S.E.M. for three determinations, where significant differences from V-PC vesicles are represented by ***P ≤ 0.001.
