TABLE 1.
Lipid composition (mol%) of synthetic reconstituted systems
Purified lipid vesicles were prepared with the lipid composition listed to analyze the potential of lipid domain formation.
| Vesicle | PC | PE | PI | PS | PA | SM | Chol |
|---|---|---|---|---|---|---|---|
| PC | 100a | — | — | — | — | — | — |
| Mix PL | 68a | 20 | 10 | 1 | 1 | — | — |
| ER | 60a | 20 | 10 | 1 | 1 | 4c | 5 |
| DRM | 42a | 18 | 1.5 | 2 | 1.5 | 12c | 23 |
| Model 1 | 100b | — | — | — | — | — | — |
| Model 2 | 80b | — | — | — | — | 5d | 15 |
| Model 3 | 50b | — | — | — | — | 20d | 30 |
| Model 4 | 30b | — | — | — | — | 30d | 40 |
—, not present; Chol, cholesterol; PI, phosphatidylinositol; PS, phosphatidylserine.
a
Phosphatidylcholine in the “natural membrane” systems was a bovine liver extract.
b
The phosphatidylcholine in the model systems was 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC).
c
Sphingomyelin in the “natural membrane” systems was porcine brain sphingomyelin.
d
In the model systems, palmitoyl-sphingomyelin (PSM) was used.