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. 2013 Nov;347(2):288–297. doi: 10.1124/jpet.113.207316

Fig. 5.

Fig. 5.

MCPIP-mediated upregulation of EC markers is associated with induction of cdh12 and cdh19 in BMNCs. BMNCs were cultured in EBM medium and transfected with MCPIP expression vector or control vector. (A) qRT-PCR was performed to determine the expression of cdh12 and cdh19 in BMNCs at the time points indicated. Relative mRNA levels were expressed as fold change compared with cells transfected with control vector. (B) Treatment of cells with siRNA against cdh12 and cdh19 inhibited MCPIP-mediated enhancement of cell attachment. (C) qRT- PCR analysis of cdh12 and cdh19 mRNA in BMNCs transfected with the expression vector for MCPIP-GFP with or without cdh12- and cdh19-specific or nonspecific siRNA showed that only siRNA specific for the particular cdh gene showed knockdown. *P < 0.05 versus MCPIP vector- or nonspecific siRNA (NS)–transfected BMNCs. (D and E) Histograms depicting the average cdh12 and cdh19 expression levels in the examined groups shown in the Western blots. (F and G) Treatment of cells with siRNA against cdh12 and cdh19 inhibited MCPIP-mediated upregulation of endothelial cell markers Tie-2 and Flk-1 in BMNCs. *P < 0.05 versus cells transfected with MCPIP only or nonspecific siRNA (NS).