Fig. 5.

PHLPP inhibitors protect cortical neurons from STS injury. Primary rat neurons were seeded onto 96-well plates. (A–C) Cells were coincubated 24 hours with PHLPP inhibitors and 50 nM STS (A), 100 nM STS (B), or 200 nM STS (C). Neuronal viability was measured by CellTiterBlue metabolism 24 hours later. (D) Cells were given a 35-minute pretreatment with PHLPP inhibitors. Drugs were removed and replaced with media containing 200 nM STS. Viability was measured by CellTiterBlue metabolism 24 hours later. Experiments were performed in replicates; n = 7 for the 250 μM NSC45586 groups in (B and C) and n = 8 for all other groups. White bars show uninjured control groups treated with DMSO only. Black bars show groups treated with STS for 24 hours. Data were analyzed by ANOVA and Newman-Keuls multiple comparison post hoc test. *Significant post hoc difference (P < 0.05) for PHLPP-inhibitor treated groups compared with injury (STS only) control. Graphs show mean + S.E.M.