Skip to main content
. 2013 Nov;347(2):276–287. doi: 10.1124/jpet.113.207449

Fig. 1.

Fig. 1.

Optimization of conditions for an intact-cell assay that is capable of high-throughput screening for small-molecule inhibitors of AC2. (A) Concentration-response curve analysis of PMA for stimulation of an AC2-mediated cAMP response in HEK-hAC2 cells. Data are the mean ± S.E.M. of three independent experiments. Inset: Inhibition of 50 nM PMA-stimulated AC2 activity with the PKC inhibitor BisI. Data are the mean ± S.E.M. of three independent experiments. (B) Evaluation of assay robustness by Z′ analysis. Data are representative of three independent experiments.