Fig. 3.

AC isoform-selectivity profile of test compounds in intact-cell studies. AC isoform selectivity was assessed by testing the ability of test compounds (30 µM) to modulate (A) 3 µM A23187-stimulated cAMP in HEK-hAC1 cells; (B) 50 nM PMA-stimulated cAMP in HEK-hAC2 cells; (C) 300 nM forskolin-stimulated cAMP in HEK-hAC5 cells; and (D) 3 µM forskolin-stimulated cAMP in HEK-wt cells. *P < 0.05; **P < 0.01 compared with vehicle condition, one-way analysis of variance followed by Dunnett’s post hoc test.