Fig. 5.

Concentration-response analysis of SKF-83566 for inhibition of cAMP. (A) Dose-response curves of SKF-83566 for inhibition of PMA-stimulated (50 nM) cAMP in HEK-hAC2 cells and forskolin-stimulated (300 nM) cAMP in HEK-hAC5 cells. (B) Dose-response curves of SKF-83566, MDL-12,330A, SQ 22,536, and 2′5′-ddAD for inhibition of PMA-stimulated (50 nM) cAMP in HEK-hAC2 cells. The SKF-83566 data in (A) and (B) are from the same experiments; these studies were performed simultaneously. Data are expressed as a percentage of the stimulation response and are reported as the mean ± S.E.M. of three independent experiments.