Fig. 6.

SKF-83566 as a chemical probe for native AC2 activity. (A) The effect of SKF-83566 on forskolin-stimulated (30 µM) cAMP was measured in mouse C2C12 skeletal muscle cells that were differentiated into myotubes. Data are mean ± S.E.M. of three independent experiments. (B) The effect of SKF-83566 on forskolin-stimulated (1 µM) IL-6 mRNA expression was measured in hBSMCs. Data are expressed as a percentage of the vehicle condition and are the mean ± S.E.M. of four independent experiments. *P < 0.05; **P < 0.01 (one sample t test compared with 100).