TABLE 2.

Effect of mutations on potentiation of glutamate-mediated Ca²⁺ mobilization by allosteric modulators

Data represent mean ± S.E.M. of at least four independent experiments performed in duplicate.

	Fold Increase in Glutamate pEC50 in Presence of 3 µM PAM
	CPPHA	VU29	DPFE	VU0364289
mGlu5-wt	3.2 ± 0.1	6.1 ± 1.4	7.7 ± 0.8	4.2 ± 0.7
F585I	1.2 ± 0.1*	11.2 ± 4.8	8.8 ± 2.1	5.6 ± 1.3
A809V	2.8 ± 0.4	2.0 ± 0.3*	2.6 ± 0.2*	1.3 ± 0.1*

^*Significantly different from mGlu₅-wt value, P < 0.05, one-way ANOVA, Dunnett’s post-test.