Fig. 4.

Expression of CAR in brain microvessels following treatment with APAP or PB. (A) Western blot analysis of microvessels isolated from rats treated with 500 mg/kg APAP or 80 mg/kg PB for 3 hours. Cytoplasmic and nuclear extracts from rat brain microvessels (10 μg) were resolved on a 10% SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride membrane. Samples were analyzed for expression of CAR. (B) Relative levels of CAR determined by densitometric analysis and the nucleus-to-cytoplasm ratio were calculated. Results are expressed as mean ± S.E.M. of three separate experiments. Asterisks represent data points that are significantly different from saline (*P < 0.05).