Fig. 7.

Effects of UVB treatments on the expression of UGT1A1 in the skin of hUGT1 mice. RNA was isolated from the skin of control and UVB-treated hUGT1 mice, and qRT-PCR was carried out for human UGT1A1 (A). Microsomes from the skin were prepared and subjected to immunoblot analysis with anti-UGT1A antibodies. As the positive control, UGT1A1 supersomes (10 µg) and human liver microsomes (10 µg) were also subjected to immunoblotting (B). The density of the bands was quantified using ImageJ Software (Wayne Rasband, NIH) (C). Error bars show S.D., n = 3. *P < 0.05.