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Rho-kinase activity as measured by phosphorylation of MYPT1 in wild-type and eNOS−/− mice. Wild-type and eNOS−/− mice were treated with either saline or fasudil. Protein was extracted from mesenteric and intestinal tissues. Rho-kinase activity was expressed as the ratio of p-MYPT1/total MYPT1. Values represent mean ± SEM. *p < 0.01 vs untreated mice.
