Figure 3. JAK1 deficient cancer cells are defective in the IFN-γ-stimulated LMP2/TAP1 antigen-processing machinery pathway.
(a) Cell lysates from 6 cell lines were analyzed by immunoblotting with an anti-JAK1 antibody. Position of the 130-kDa full-length JAK1 is indicated. Other bands appear on the immunoblot are unknown non-specific immunoreactivities. (b–c) Cells were treated with IFNγ for the indicated time. Cell lysates were analyzed by immunoblotting for IFN-γ-induced pSTAT1, IRF1, LMP2, and TAP1.