Table 1. Recombinant endochitinase purification results.

Purification step	Total volume (mL)	Total protein (mg)	Total activity (U)	Specific activity (U/mg)	Purification fold	Yield (%)
Culture broth	30	77	693	9.0	1.0	100
(NH4)2SO4 precipitation	12	46	598	13	1.4	86
DE-52 anion-exchange chromatography	2.6	4.6	244	53	5.9	35
Sephadex G-100 size-exclusion chromatography	1.0	1.6	113	71	7.9	16