Fig 6.
Inhibitory effect of the nonbactericidal sample. (A) The standard assay to test bactericidal activity against SL1344 was carried out using PBS, individual bactericidal samples (B), the nonbactericidal sample (NB; results from multiple experiments), or a mix of individual bactericidal samples with the nonbactericidal sample (B + NB). In some of the experiments, individual bactericidal samples were mixed with the nonbactericidal sample, which had been preabsorbed with either S. Typhimurium SL1344 (B + NBSt) or S. Dublin 2229 (B + NBSd). *, P = 0.0022. The dashed line indicates the starting inoculum. (B) Bactericidal (B) and nonbactericidal (NB) samples were analyzed by ELISA to determine levels of IgM and IgG antibodies reactive with S. Typhimurium LPS.