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. 2013 Oct;20(10):1569–1577. doi: 10.1128/CVI.00305-13

Fig 1.

Fig 1

Identification and purification of the target Fasciola antigen. (A) Silver-stained SDS-PAGE. (B) Western blot for different antigenic sources of F. gigantica, S. mansoni, and A. lumbricoides using rabbit anti-27-kDa Fasciola IgG antibody. (A and B) Lane 1, F. gigantica adult worm antigen preparation (FWAP); lane 2, excretory/secretory (E/S) products from F. gigantica; lane 3, S. mansoni adult worm antigenic preparation (SWAP); lane 4, A. lumbricoides adult worm antigenic preparation (AWAP); lane 5, the purified 27-kDa antigen. The developed anti-Fasciola antibody identified its target epitope at 27 kDa in FWAP, E/S products, and the purified antigen (lanes 1, 2, and 5) but not in SWAP and AWAP (lanes 3 and 4). (C) Inhibition Western blot for different antigenic sources of F. gigantica by using rabbit anti-27-kDa Fasciola IgG antibody saturated with purified 27-kDa antigen. Lane 1, FWAP immunostained with the anti-Fasciola antibody; lane 2, FWAP immunostained with the saturated antibody; lane 3, E/S products immunostained with the anti-Fasciola antibody; lane 4, E/S products immunostained with saturated antibody. The saturated anti-Fasciola antibody did not identify its target epitope at 27 kDa in FWAP and E/S products (lanes 2 and 4). Molecular mass standards (St.) included were phosphorylase B (97.4 kDa), bovine serum albumin (66.2 kDa), glutamate dehydrogenase (55 kDa), ovalbumin (42.7 kDa), aldolase (40 kDa), carbonic anhydrase (31 kDa), and soybean trypsin inhibitor (21.5 kDa). (D) The 27-kDa antigen purified from serum of an F. gigantica-infected individual showing a single peak, at an absorbance of 200 nm, at 5.9 min, using CZE.