Figure 1.

Potency and Eph receptor selectivity of compounds 2 and 5. The left panels show curves for inhibition of ephrin-B2 AP binding to immobilized EphB4 Fc by compounds 2 and 5 and the calculated IC₅₀ values. The right panels show the levels of bound ephrin-A5 AP (for EphA Fc receptors) or ephrin-B2 AP (for EphB Fc receptors) in the presence of 200 µM compound 2 or 100 µM compound 5 normalized to the levels in the absence of compound. Error bars represent standard errors from duplicate measurements (compound 2) or triplicate measurements (compound 5). The curves for IC₅₀ determination were repeated in 3 independent experiments for compound 2 and in 4 independent experiments for compound 5 (see Table 1). The Eph receptor selectivity experiments were repeated twice with similar results.