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Decrease in the specific signal for LAM over time in spiked serum by membrane insertion assay. Black triangles and black grey line indicate positive signal for detection of the antigen in the serum of LAM spiked human serum incubated for 0 and 6 h. LAM was undetectable after overnight (>16 hrs.) incubation. Light gray line indicated the measure of non-specific binding of the reporter antibody with the control serum.
