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. Author manuscript; available in PMC: 2013 Oct 25.
Published in final edited form as: Electrophoresis. 2012 Jul;33(12):10.1002/elps.201200006. doi: 10.1002/elps.201200006

Table 3.

A Major plasma proteins eluted from the first column after detachment from the three-column system (see Figure 4A) identified by LC-MS/MS. 4M NaCl in 10 mM Tris-HCl, pH 7.4 was used as Buffer A.
Acc. No. Protein name Score Uniq.
pept.
IPI00783987.2 Complement C3 2825.45 54
62739186 Complement factor H 2424.82 46
IPI00019580.1 Plasminogen 1927.69 42
IPI00478003.3 Alpha-2-macroglobulin 1806.31 37
IPI00298497.3 Fibrinogen 1677.13 32
IPI00022229.2 Apolipoprotein B 1661.45 36
IPI00745872.2 Human serum albumin 1546.10 29
IPI00892547.1 Complement component 4A 1444.19 26
IPI00797833.3 LMW Kininogen=1 1365.61 24
IPI00867588.1 Fibronectin 1211.96 23
B Major plasma proteins eluted from the second column after detachment from the three-column system (see Figure 4A) identified by LC-MS/MS. 4 M NaCl in 10 mM Tris-HCl, pH 7.4 was used as Buffer A.
Acc. No. Protein name Score Uniq.
pept.
IPI00783987.2 Complement C3 3951.21 73
IPI00022229.2 Apolipoprotein B 2765.35 59
IPI00892547.1 Complement component 4A 2079.37 38
IPI00478003.3 Alpha-2-macroglobulin 2061.22 40
IPI00029739.5 Complement factor H 1955.98 37
IPI00797833.3 LMW Kininogen=1 1744.34 32
IPI00745872.2 Human serum albumin 1463.63 30
IPI00298497.3 Fibrinogen 1369.93 25
IPI00019580.1 Plasminogen 1234.44 26
IPI00021841.1 Apolipoprotein A-1 1177.26 21
C Major plasma proteins eluted from the third column after detachment from the three-column system (see Figure 4A) identified by LC-MS/MS. 4M NaCl in 10 mM Tris-HCl, pH 7.4 was used as Buffer A.
Acc. No. Protein name Score Uniq.
pept.
I225131003 Apolipoprotein B 4801.35 97
IPI00783987.2 Complement C3 4688.66 89
IPI00892547.1 Complement component 4A 2357.27 46
IPI00478003.3 Alpha-2-macroglobulin 2010.13 39
IPI00215894.1 LMW Kininogen-1 1777.46 33
IPI00029739.1 Complement factor H 1713.16 34
IPI00745872.2 Human serum albumin 1571.07 32
IPI00032291.2 Complement C5 1380.07 30
IPI00021841.1 Apolipoprotein A-1 1310.37 25
IPI00654888.4 Plasma kallikrein 1174.02 21
Other highly enriched, potentially interested, physiologically active proetins
  1. Clotting factor XI (highly enriched)
  2. Hyaluronan-binding protein 2 (highly enriched)
  3. lalp heavy chain 4
  4. Clotting factor V
  5. Clotting factor XII
Other highly enriched, potentially interested, physiologically active proteins:
  1. Protein S
  2. Clotting factor V
  3. Clotting factor XII
Other highly enriched, potentially interested, physiologically active proteins
  1. lalp heavy chain 4
  2. Prothrombin
  3. Clotting factor V
  4. Clotting factor XI
  5. Clotting factor IX
  6. Protein S
  7. Clotting factor XII