Table 3.
| A Major plasma proteins eluted from the first column after detachment from the three-column system (see Figure 4A) identified by LC-MS/MS. 4M NaCl in 10 mM Tris-HCl, pH 7.4 was used as Buffer A. | |||
|---|---|---|---|
| Acc. No. | Protein name | Score | Uniq. pept. |
| IPI00783987.2 | Complement C3 | 2825.45 | 54 |
| 62739186 | Complement factor H | 2424.82 | 46 |
| IPI00019580.1 | Plasminogen | 1927.69 | 42 |
| IPI00478003.3 | Alpha-2-macroglobulin | 1806.31 | 37 |
| IPI00298497.3 | Fibrinogen | 1677.13 | 32 |
| IPI00022229.2 | Apolipoprotein B | 1661.45 | 36 |
| IPI00745872.2 | Human serum albumin | 1546.10 | 29 |
| IPI00892547.1 | Complement component 4A | 1444.19 | 26 |
| IPI00797833.3 | LMW Kininogen=1 | 1365.61 | 24 |
| IPI00867588.1 | Fibronectin | 1211.96 | 23 |
| B Major plasma proteins eluted from the second column after detachment from the three-column system (see Figure 4A) identified by LC-MS/MS. 4 M NaCl in 10 mM Tris-HCl, pH 7.4 was used as Buffer A. | |||
|---|---|---|---|
| Acc. No. | Protein name | Score | Uniq. pept. |
| IPI00783987.2 | Complement C3 | 3951.21 | 73 |
| IPI00022229.2 | Apolipoprotein B | 2765.35 | 59 |
| IPI00892547.1 | Complement component 4A | 2079.37 | 38 |
| IPI00478003.3 | Alpha-2-macroglobulin | 2061.22 | 40 |
| IPI00029739.5 | Complement factor H | 1955.98 | 37 |
| IPI00797833.3 | LMW Kininogen=1 | 1744.34 | 32 |
| IPI00745872.2 | Human serum albumin | 1463.63 | 30 |
| IPI00298497.3 | Fibrinogen | 1369.93 | 25 |
| IPI00019580.1 | Plasminogen | 1234.44 | 26 |
| IPI00021841.1 | Apolipoprotein A-1 | 1177.26 | 21 |
| C Major plasma proteins eluted from the third column after detachment from the three-column system (see Figure 4A) identified by LC-MS/MS. 4M NaCl in 10 mM Tris-HCl, pH 7.4 was used as Buffer A. | |||
|---|---|---|---|
| Acc. No. | Protein name | Score | Uniq. pept. |
| I225131003 | Apolipoprotein B | 4801.35 | 97 |
| IPI00783987.2 | Complement C3 | 4688.66 | 89 |
| IPI00892547.1 | Complement component 4A | 2357.27 | 46 |
| IPI00478003.3 | Alpha-2-macroglobulin | 2010.13 | 39 |
| IPI00215894.1 | LMW Kininogen-1 | 1777.46 | 33 |
| IPI00029739.1 | Complement factor H | 1713.16 | 34 |
| IPI00745872.2 | Human serum albumin | 1571.07 | 32 |
| IPI00032291.2 | Complement C5 | 1380.07 | 30 |
| IPI00021841.1 | Apolipoprotein A-1 | 1310.37 | 25 |
| IPI00654888.4 | Plasma kallikrein | 1174.02 | 21 |
- Clotting factor XI (highly enriched)
- Hyaluronan-binding protein 2 (highly enriched)
- lalp heavy chain 4
- Clotting factor V
- Clotting factor XII
- Protein S
- Clotting factor V
- Clotting factor XII
- lalp heavy chain 4
- Prothrombin
- Clotting factor V
- Clotting factor XI
- Clotting factor IX
- Protein S
- Clotting factor XII