Figure 3. Cellular ROS generation is influenced by the mtDNA in cardiomyocytes.

A) Cardiomyocytes isolated from control (C57ⁿ:C57^mt and C3Hⁿ:C3H^mt) and MNX (C57ⁿ:C3H^mt and C3Hⁿ:C57^mt) mouse hearts were treated with DCF-DA and cellular fluorescence measured (485 nm/530 nm - excitation/emission) B) Bar graph illustrating fluorescence relative to the C57ⁿ:C57^mt controls. The nuclear genome for each group is indicated on the X-axis, whereas the filled or open bars indicate the presence of either the C57 mtDNA or C3H mtDNA, respectively. Data = mean ± SEM from 35–50 cardiomyocytes. An asterisk (*) indicates a significant difference (p < 0.001) exists compared to nuclear genome matched control.