Fig 3.

Binding and activation of the PDGF β receptor. Detergent extracts were prepared from BaF3 cells expressing the wild-type PDGF β receptor or PR-L512I and coexpressing either the empty vector (v), the wild-type E5 protein (E5), E5.LRM19 (19), or E5.LRM4 (4). Samples were immunoprecipitated (IP) with antibody recognizing the PDGF β receptor (R) or the E5 protein (E5) and then immunoblotted (Western blot [WB]) with antibody recognizing phosphotyrosine (PY), the PDGF β receptor, or the E5 protein. m and p indicate the mature and precursor forms of the PDGF β receptor, respectively.