Fig 8.

Mapping of mutations that confer activity with PR-L512I. (A) BaF3 cells expressing the βαβ chimera (black bars), the wild-type PDGF β receptor (gray bars), or PR-L512I (white bars) were infected with retroviruses expressing no insert (vec), the wild-type E5 protein, or the indicated E5 mutant. Viable cells were counted and recorded as described in the legend of Fig. 2. Statistical significance of the results of the PR-L512I tests compared to the E5/PR-L512I combination is indicated by three asterisks (P ≤ 0.002). (B) BaF3 cells expressing PR-L512I were infected with retroviruses expressing the indicated E5 mutant and analyzed as described above for panel A. The graph shows the averaged results of multiple independent experiments.