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Initiation of HSV-1 replication in dystonin-depleted and control cells. (A) shGFP or shDyst HFFF2 cells were infected with 5 PFU/cell of tsK/luci and incubated for 2 h, 4 h, or 7 h at a permissive temperature (31°C). Cells were then harvested and lysed and luciferase activity was measured. Dashed line, background level. (B) Data shown in panel A are represented as a graph to show the rate of increase in luciferase levels with time after infection. mi, mock infected.
