Fig 3.

Onset of viral protein production in dystonin-depleted and control cells assessed by FACS analysis. (A) Mock-infected shControl cells or shControl or shDyst HFFF2 cells infected with 5 PFU/cell of WT HSV-1 were harvested at 2 h, 4 h, or 6 h after infection, fixed, permeabilized, and incubated with antibody 11060 against ICP0 and GAM₄₈₈. Cells were then analyzed by flow cytometry. The numbers of fluorescing cells and their fluorescence intensities were determined for mock-infected shControl cells (black line), infected shControl cells (blue line), or infected shDyst cells (red line). The percentages of the total cell population identified as positive (i.e., above the background level, as defined by the fluorescence intensity of the mock-infected shControl cells) are indicated using the same color coding. (B) Summary of mean fluorescence intensities indicated for panel A. The number of cells analyzed is given for each condition. Dashed line, background level defined by the fluorescence intensity of mock-infected shControl cells.