Fig 4.

Syndecan expression levels and baculovirus binding in various mammalian cell types. (A) 293T, MG-63, HepG2, and EA.hy926 cells were immunostained with rabbit anti-SDC-1 to -4 antibodies and anti-rabbit Alexa 488 antibody and imaged by confocal microscopy (60× magnification). Image analysis was performed as described in Materials and Methods. Mean fluorescence values and standard deviations are shown. (B) The amount of baculovirus binding at the surface of 293T, MG-63, HepG2, and EA.hy926 cells. The virus (MOI, 500) was allowed to attach on the surface of the cells. The bound virus was stained with rabbit anti-baculovirus antibody and anti-rabbit Alexa 488 antibody and imaged by confocal microscopy (60× magnification). Image analysis was performed as described in Materials and Methods. Mean fluorescence values and standard deviations are shown.