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. 2013 Oct;87(20):11076–11087. doi: 10.1128/JVI.01425-13

Fig 2.

Fig 2

Assay optimization. (A) 293T cells returned the highest activity levels for both reporter constructs. Cells were infected individually with recMeV-ren or transfected with the IAV minigenome reporter and superinfected with IAV-WSN. RLU values represent means and SD for four experiments. (B) Efficient expression of both luciferase reporters in coinfected 293T cells. Cells transfected with the IAV-firefly luciferase plasmid were superinfected with IAV-WSN and increasing amounts of recMeV-ren, ranging from 0.05 to 0.2 infectious unit/cell. Relative activities of either reporter were determined at 30 hpi. Controls lacked either recMeV-ren or IAV-WSN. Values represent means and SD for three experiments. (C) The highest IAV-firefly luciferase reporter expression levels were achieved with swine-origin IAV-Texas. Cells transfected with IAV-firefly luciferase minigenome plasmid DNA were infected with different IAV strains at an MOI of 0.1 infectious unit/cell. Luciferase activities were determined at 30 hpi. Values represent means and SD for three experiments.