Fig 4.

Dual myxovirus screen of a 10,000-entry diversity set. (A) Schematic of primary, confirmatory, and counterscreens for hit identification. (B) Normalized, scaled, and ordered screening scores (Z scores) obtained for each target virus. Lines mark hit selection cutoffs; selected hit candidates are shown to the right of the dashed lines. (C) Selected hit candidates plotted by the Z score obtained for each target virus. Dashed blue lines mark the cutoffs for MeV (approximately 2.5 SD above the mean) and IAV-Texas (approximately 2 SD above the mean). Red symbols mark compounds with activity against both myxovirus targets. (D) Venn diagram summarizing the second-tier confirmatory screen of all 360 primary hit candidates shown in panel C against recRSV-ren (hit cutoff, 2 SD above the mean).