Table 1.
Comparison of assay formats used in this study
| Plate format | Target virusa | Z′ valueb | S/B ratioc | % CV |
|---|---|---|---|---|
| 96 wells | recMeV-ren | 0.74 | 31 | 8.0 |
| IAV-firefly luciferase | 0.79 | 109 | 6.7 | |
| IAV-Texas | ||||
| 384 wells | recMeV-ren | 0.74 | 41 | 8.2 |
| IAV-firefly luciferase | 0.67 | 89 | 10.9 | |
| IAV-Texas |
a
Cryopreserved 293T cells transfected with IAV-firefly luciferase were plated and coinfected with recMeV-ren and IAV-Texas in the presence of the pan-myxovirus inhibitor JMN3-003 (final concentration, 1 μM) or an equivalent amount of vehicle (DMSO). Relative luciferase unit values were determined at 30 hpi.
b
Z factor (43). Statistical analyses are based on means for four independent experiments.
c
Ratio of signal to background.