Fig 2.

VP11/12 interacts with p85, Lck, Shc, and Grb2. Jurkat E6-1 cells were mock infected or infected with a KOS37-derived virus expressing C-terminally GFP-tagged VP11/12 (KOS37-UL46 GFP), a KOS37-derived virus expressing N-terminal tagged VP11/12 (KOS37-GFP UL46), or a KOS-derived virus expressing GFP (KOS-G) for 13 h. Whole-cell lysates (WCL) were immunoprecipitated using an antibody against GFP (IP: GFP) and analyzed via Western blotting (WB) for p85, Lck, Shc, Grb2, GFP, and ICP27 (HC, heavy chain).