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Association between VP11/12 and Shc requires the NPLY motif at position 657. (A) Extracts of Jurkat E6-1 cells infected with the indicated viruses (WCL) were precipitated with an anti-GFP antibody (IP: GFP) and analyzed by Western blotting (WB) as described for Fig. 3A. (B) Extracts of Jurkat E6-1 cells infected with KOS37-UL46 GFP or KOS37-UL46 GFP Y657F were analyzed in a GST pulldown assay using GST or GST-Shc PTB as for Fig. 3B.
