Table 2.
Primers used for generating point mutated KOS37-UL46 viruses
| Primer | Sequence (5′–3′)a |
|---|---|
| Y519F-JRS 812/JRS 813 | CCGAGCCCCCGCTGCGGCCACACAGCCGGCCACGTATTTCACGCACATGGGGGAGGTGCtagggataacagggtaatcgattt |
| ACGGGCCGGGAGGCGCGGGGGCACCTCCCCCATGTGCGTGAAATACGTGGCCGGCTGTGTGgccagtgttacaaccaattaacc | |
| Y613F-JRS 846/JRS 847 | CGCGAACGGCACGCCCCCTACGAGGACGACGAGTCAATATTCGAGACGGTGAGCGAGGACGtagggataacagggtaatcgattt |
| TTCCTCGTAGACACGCCCCCCGTCCTCGCTCACCGTCTCGAATATTGACTCGTCGTCCTCGgccagtgttacaaccaattaacc | |
| Y624F-JRS 687/RS 688 | GTCAATATACGAGACGGTGAGCGAGGACGGGGGGCGTGTCTTCGAGGAAATACCATGGATGCtagggataacagggtaatcgattt |
| GCAGACGTTTTCGTAGACCCGCATCCATGGTATTTCCTCGAAGACACGCCCCCCGTCCTCGgccagtgttacaaccaattaacc | |
| Y613F/Y624F-JRS 846/JRS 848 | CGCGAACGGCACGCCCCCTACGAGGACGACGAGTCAATATTCGAGACGGTGAGCGAGGACGtagggataacagggtaatcgattt |
| TTCCTCGAAGACACGCCCCCCGTCCTCGCTCACCGTCTCGAATATTGACTCGTCGTCCTCGgccagtgttacaaccaattaacc | |
| Y633F-JRS 760/JRS 761 | GGGGGGCGTGTCTACGAGGAAATACCATGGATGCGGGTCTTCGAAAACGTCTGCGTGAACAtagggataacagggtaatcgattt |
| GGCCGGCGCTGCATTCGCCGTGTTCACGCAGACGTTTTCGAAGTCCCGCATCCATGGTATTgccagtgttacaaccaattaacc | |
| Y657F-JRS 810/JRS 811 | GCGCCGGCCTCCCCGTACATTGAGGCGGAAAATCCCCTGTTCGACTGGGGGGGATCCGCCCtagggataacagggtaatcgattt |
| GCGGCCCGGGGGGGAAAATAGGGCGGATCCCCCCCAGTCGAACAGGGGATTTTCCGCCTCAgccagtgttacaaccaattaacc |
a
HSV-1 sequences are capitalized, point mutations are in bold, and I-SceI-aphAI sequences are in lowercase.