Fig 3.

An adoptive-transfer model to detect virus-specific T cells in the lung. (A) Illustration of the adoptive-transfer model. P14.Thy1.1 spleen cells containing 1 × 10⁶ naive Vα2⁺ CD8⁺ CD3⁺ T cells were adoptively transfused into Thy1.2⁺ C57BL/6 recipients (P14 chimeric mice) which were infected 3 days later (d0) with 100 to 200 PFU of rPVM-GFPgp33. (B) Weight curves after infection of C57BL/6 and P14 chimeric mice with rPVM-GFPgp33. Data are means and standard deviations of 4 to 14 mice per time point pooled from 3 independent experiments. (C) Pulmonary virus titers at the indicated time points after infection. Data are from C57BL/6 mice and P14 chimeric mice gained in two different experiments. (D) Percentage of gp33 dimer-positive cells among total CD3⁺ CD8⁺ BAL fluid cells of P14 chimeric mice that had been infected with 100 to 200 PFU of rPVM-GFPgp33 8 to 12 days previously. (E) Percentage of Thy 1.1-positive cells among total BAL fluid or lung parenchyma CD3⁺ CD8⁺ T cells responding with IFN-γ production to stimulation with gp33 peptide. The means and SEMs from pooled data of three independent experiments are shown. n.s., not significant; ***, P < 0.0001.